猪LIAS基因微环表达载体的构建

doi:10.11843/j.issn.0366-6964.2014.03.006

Abstract

Abstract:

In order to construct a long-term and high-level transgene expression recombinant vector，LIAS gene was cloned from the muscle of porcine by RT-PCR and inserted into minicircle plasmid.The minicircle-LIAS vector was transfected into HeLa cells to investigate the duration of GFP expression.The results showed that the LIAS gene which was 1 119 bp length was completely cloned and inserted into minicircle plasmid.The vector was transfected into HeLa cells and the duration of GFP expression of recombinant minicircle-LIAS vector was obviously higher than that of pEGFP-N1 plasmid.This result provides a new thought and experimental material for the process of LIAS biological synthesis and function regulation.

CLC Number:

S828
S813.3

GAO Hui-zhen，WANG Xiao-ting,GUO Yu-jie，HAN Li-qiang,LU Wei-fei,YANG Guo-yu. Construction of Minicircle Vector Carrying Porcine LIAS Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(3): 380-384.

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Construction of Minicircle Vector Carrying Porcine LIAS Gene

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